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. 2013 May 16;8(5):e63613. doi: 10.1371/journal.pone.0063613

Figure 1. Interleukin-10 expression is upregulated following ovalbumin stimulation in Dll4-pretreated dendritic cells.

Figure 1

(A) Cytotoxicity effect of Dll4 on dendritic cells. On Day 4 of culture, bone marrow-derived dendritic cells (DCs) were treated with 1 and 5 µg/mL of Dll4 for 48 h, then cultured with or without 100 µg/mL ovalbumin (OVA) for an additional 24 h. Cell viability was detected using an MTT assay. Untreated DCs were pulsed with OVA or medium alone as the controls. (B) Interleukin (IL)-10 and IL-12 mRNA expression and (C) protein levels from various doses of Dll4-pretreated DCs with OVA stimulation. On Day 4 of culture, bone marrow-derived DCs were treated with medium or various concentrations of Dll4 (1 and 5 µg/mL) for 48 h and then activated by OVA (100 µg/mL) stimulation for an additional 5 or 24 h. Untreated immature DCs served as the control group (DC). Transcripts and protein production of IL-10 and IL-12 were measured using a quantitative real-time RT-PCR and ELISA, respectively. Results from 3 independent experiments are shown and expressed as the mean ± SEM. ** p<0.01, *** p<0.001 compared to OVA-treated DCs (OVA group).