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. 2013 May 16;8(5):e63613. doi: 10.1371/journal.pone.0063613

Figure 3. Dll4 pretreatment affects the phenotype of ovalbumin-stimulated dendritic cells.

Figure 3

Dll4-pretreated or untreated immature dendritic cells (DCs) were or were not stimulated with ovalbumin (OVA) for 24 h. After incubation, cells were collected, and expression of MHC class II, CD40, CD54, CD80, and CD86 by DCs were analyzed using flow cytometry. DCs stimulated with only OVA (OVA group) were used as the positive control. (A) Values shown in the flow cytometric profiles are the mean fluorescence intensity (MFI). Values shown were from one representative experiment of 3 independent experiments performed. (B) The MFI was calculated, and results are expressed as the mean ± SEM from 3 independent experiments. * p<0.05, **p<0.01, ***p<0.001 compared to OVA-treated DCs (OVA group).