Figure 6. Breakdown of long PFL by eNOS and PKG inhibitors.

A: the changes in [Ca²⁺]_i level (black curve) and NO accumulation in DAF-FM (ΔF/F₀, blue curve) upon the application of 5 µM ACh after cell preincubation within 5 minutes with 5 µM inhibitor of PKG, KT5823, in 37±8,6% of cells, (n = 93). B: the effect of of PKG inhibitor, KT5823 (5 µM; curve 1), and KT5823+ inhibitor of NOS, L-NAME (1 mM; curve 2) added 5 minutes before the start of calcium dynamics registration in the cytosol, (n = 137). Slow increase of [Ca²⁺]_i occurs in 51±19,4% of the cells (curve 1) and in 35±8,0% of cells (curve 2). C: the effect of PKG inhibitor, Rp-8-Br-cGMPS, (10 µM) which prevents the onset of periodic modes in all cells and results in a rise of Ca²⁺ in the cytosol in 30±4,5% of adipocytes (curve 1). The application of 5 µM ACh against a background of Rp-8-Br-cGMPS and NOS inhibitor, 7-NI (1 µM) leads to slow rise of Ca²⁺ in the cytosol in 11±3,2% of adipocytes (curve 2), n = 214.