Figure 8. The induction of various modes of [Ca²⁺]_i oscillations in adipocytes by the intermediates of long PFL(3).

A: upon application of 5 µM SNAP, a donor of NO, the generation of fast Ca²⁺ oscillations occurs in 34±9,6% of the cells (black curve) and slow high-amplitude Ca²⁺ oscillations (blue curve) are observed in 11±2,5% of adipocytes, (n = 208). B: the transition of the system to a steady oscillatory mode with a high mean value of Ca²⁺ for the oscillation period in 15±3,6% of the cells, (n = 97). C: the transition of the system to a new stable stationary state with a high level of calcium in 59±9,8% of the cells, (n = 105). D: [Ca²⁺]_i oscillations with a period of several dozen seconds are induced in 43±6,1% of the cells by the application of 0,1 µM 8Br-cGMP, a permeating analog of cGMP, and are quickly suppressed by an inhibitor of ADPRC – Nicotinamide (NAM), (n = 132). E: [Ca²⁺]_i oscillations with varying period of oscillations are induced in 26±11,0% of adipocytes by the application of 0.1 µM 8Br-cGMP and are quickly suppressed by NAM, (n = 116). F: oscillations caused by the application of 1 µM βNAD are observed in 57±10,3% of adipocytes and are quickly suppressed by nicotinamide (NAM) (n = 163). There are no [Ca²⁺]_i oscillations in 43±8,6% of the cells (grey curve).