Figure 2. Different kinds of phenazine production and its influence in eDNA release. Comparison of bacterial colony formation on LB agar plate after 48 h incubation with non-filtered (upper half) and filtered (bottom half) bacterial supernatant (A). Production of various kinds of phenazine molecules by P. aeruginosa PAO1 strains (B). Quantification of eDNA release in supernatants of various kinds of phenazine producing P. aeruginosa PAO1 (C) and PA14 (D) strains. Error bars represents standard deviations from the mean (n = 3). Asterisks indicate statistically significant (p < 0.05) differences in eDNA concentration in comparison to the PAO1 wildtype (C) and mutant strain ∆phzA-G (D). Schematic represents the relationship between eDNA release and phenazine production is bridged via H₂O₂ generation and subsequent H₂O₂ mediated cell lysis (E).