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. 2013 May 16;8(5):e63388. doi: 10.1371/journal.pone.0063388

Figure 4. MIYAIRI 588 suppressed hepatic oxidative stresses and induced nuclear factor erythoid 2-related factor 2 expression.

Figure 4

Rats were fed a control (CSAA) diet, CDAA diet, or CDAA diet plus MIYAIRI 588 for 8 weeks. MIYAIRI 588 was administered after CDAA diet feeding for 2 weeks. Cont, control. (A) 4-HNE-stained sections of liver specimens have been shown. The data are representative of 6 individual liver sections. Original magnification, ×40. Quantitative analysis of 4-HNE protein adducts was performed by counting the 4-HNE-positive cells for every 5 centrilobular areas of the liver tissue sections obtained for each group (lower panel). (B) Hepatic MDA levels were measured in 3 groups. The data show mean ± SD values. **p<0.01 vs. the CDAA-diet-fed group. (C) Hepatic superoxide generation was detected with dihydroethidium staining in 3 groups. (D) Immunostaining of Nrf2 was shown in the liver of the rats of each group. Data are representative of 6 individual liver sections. Original magnification, ×40 (upper panels) or ×100 (lower panels). (E) Expression of Nrf2 and its targeted genes encoding enzymes, such as HO-1, NQO1, and BSEP, was examined by western blot analysis in the liver samples of each group. β-actin expression was analyzed as a loading control.