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. 2013 May 16;8(5):e63388. doi: 10.1371/journal.pone.0063388

Figure 8. MIYAIRI 588 prevents the progression of CDAA-diet-induced liver fibrosis and cirrhosis.

Figure 8

Male Fischer 344 rats (n  = 6 per group) were fed a CSAA diet (Cont), CDAA diet, or CDAA diet plus MIYAIRI 588 for 8 or 16 weeks. MIYAIRI 588 was administered after CDAA diet feeding for 2 weeks. (A) Hepatic fibrosis was assessed by Azan-Mallory staining. Data are representative of 6 individual liver sections. Original magnification, ×40. The fibrosis area was assessed using image analysis techniques to calculate the ratio of connective tissue to the whole area of liver sections stained with Azan-Mallory. The data are expressed as mean ± SD values. ** p<0.01 compared with the CDAA-diet-fed group. (B) Immunostaining of α-SMA expression is shown. Data are representative of 6 individual liver sections. Original magnification, ×40. (C) α-SMA and collagen I expression was examined by western blot analysis at the indicated time points. β-actin expression was used as the loading control.