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. 2013 May 16;8(5):e63388. doi: 10.1371/journal.pone.0063388

Figure 9. MIYAIRI 588 inhibits development of CDAA-diet-induced hepatocarcinogenesis.

Figure 9

Rats were fed a CSAA diet (Cont), CDAA diet, or CDAA diet plus MIYAIRI 588 for 8 or 16weeks. MIYAIRI 588 was administered after CDAA diet feeding for 2 weeks. (A) Representative GST-P-positive preneoplastic foci in the liver of rats were shown. The GST-P-positive area was assessed by calculating the ratio of GST-P foci to the whole area of liver sections (lower panels). Data are representative of 6 individual liver sections. The data are expressed as mean ± SD values. Scale bars were indicated in each photograph. **p<0.01 compared with the CDAA-diet-fed group. (B) GST-P expression was detected by western blot analysis at the indicated time points. β-actin expression was used as the loading control. (C, D) Tumor number (≥1.0 mm) and maximal tumor size (diameter in mm) in the livers of rats fed on a CDAA diet (n  = 8) and CDAA diet plus MIYAIRI 588 (n  = 9) rats. Cont, control. ND, not detected. The data are expressed as mean ± SD values. *p<0.05, **p<0.01 compared with the CDAA-diet-fed group.