Figure 6. Delayed pathogen-induced disease symptom represented in M18 protease subfamily-silenced plant.

A. Disease symptom of M18 protease subfamily-silenced N. benthamiana plant following bacterial host pathogen infection (P. syringae pv. tabaci, OD₆₀₀ = 0.005). Disease symptoms were taken at 3 dpi (upper panel). Trypan blue staining is in the same area as the photographs were taken at 3 dpi (lower panel). Scale bars = 0.3 mm. B. Relative intensity of trypan blue stained N. benthamiana plants. Relative intensity was calculated to the control. Values are means±SD (n = 3). C. Bacterial cell growth in control and M19 protease subfamily-silenced N. benthamiana plants infiltrated with P. syringae pv. tabaci (1×10⁸ CFU ml⁻¹ in MgCl₂) was determined at 0, 1 and 2 dpi. Values are means±SD (n = 3). Different letters indicate significant differences at the 95% level by Duncan’s multiple range tests. D. Transcript level of M18 protease subfamily member was examined in Ncn10583- silenced plant. Values are means±SD (n = 3). The values were normalized to NbActin and were calculated to the control. Similar results were obtained from at least two experiments. One representative experiment is shown. Asterisks indicate significant differences relative to the control as determined by Student’s t test (*P<0.05, **P<0.01, ***P<0.001).