Table 3. Means of IFN-γ, IL-4 and IL-10 levels following stimulation with P1, P2, P3 and P4 peptide mixture (polytope).

Leishmanin skin test induration/mm IFN-γ pcm/ml IL-4 pcm/ml IL-10 pcm/ml
All volunteers (n = 10)
Control (No antigens/peptides) 242 ± 266 (181) 24 ± 36 (6) 624 ± 496 (472)
Soluble Leishmania antigen 194 ± 242 (131) 18 ± 24 (14) 915 ± 508 (934)
P1, P2, P3, P4 peptides mixture 249 ± 321 (177) 28 ± 34 (17) 285 ± 315 (196)*
LST-reactive (≥ 5 mm)(n = 4)
Control (No antigens/peptides) 47 ± 94 (0.5) 0.5 ± 0.6 (0.5) 506 ± 445 (433)
Soluble Leishmaniaantigen 25 ± 28 (11.5) 3.8 ± 3.8 (3.5) 906 ± 588 (875)
P1, P2, P3, P4 peptides mixture 21 ± 22 (16.5) 9 ± 16 (1.5) 79 ± 36 (68)**___
LST non-reactive ( = 0 mm)(n = 6)
Control (No antigens/peptides) 372 ± 267 (304) 40 ± 40 (25) 702 ± 554 (472)
Soluble Leishmania antigen 307 ± 259 (245) 27 ± 27.3 (21) 921 ± 507 (940)
P1, P2, P3, P4 peptides mixture 402 ± 340 (213) 40 ± 38 (28) 423 ± 347 (315)

Continuous variables are expressed as mean ± SD (median). pcm/ml = picogram/ml. P1, P2, P3 and P4 = the predicted and synthesized peptides. *p = 0.004 ** p = 0.00.