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. 2013 May 16;8(5):e62852. doi: 10.1371/journal.pone.0062852

Figure 1. Mutation events in malignant glioma alter PTPRK phosphatase activity and post-translational processing.

Figure 1

(A) Wild type PTPRK (PTPRK-wt) and its variants were transiently re-expressed in U87-MG cells. Effect of PTPRK mutations on phosphatase activity is shown. Values are mean ± standard error of 5 assays in each group. Multiple comparisons were performed to determine activity differences between the variants. P<0.05 was considered significant for ANOVA analysis. (B) Western blot of lysates from U87-MG mock cells and cells expressing wild type PTPRK or indicated PTPRK mutations. Immunoblotting was performed using an antibody against the PTPRK extracellular domain, recognizing full length PTPRK (180-kDa) and processed extracellular fragment of size 120-kDa. Immunoblot was reprobed for GAPDH as a loading control.