Table 1. Preferential interaction coefficients of free and associated proteins in 6 molal glycerol.
D1.3 | lysozyme | D1.3-lysozyme | D44.1 | Lysozyme | D44.1-lysoyzme | |
−11.1±0.4 | −5.2±0.8 | −20.6±1.5 | −28.2±1.5 | −6.9±0.8 | −33.5±1.9 | |
a | −4.3±1.7 | 1.7±2.5 | ||||
b | −9.2±0.4 | −3.5±0.5 | −13.9±1.3 | −26.0±1.6 | −4.7±0.7 | −31.2±1.7 |
a | −1.2±1.5 | −0.5±2.4 | ||||
b | −1.9±0.2 | −1.8±0.2 | −6.7±0.5 | −2.1±0.4 | −2.2±0.4 | −2.3±0.6 |
a | −3.0±0.6 | 2.0±0.9 | ||||
c | −2.3±0.1 | 1.2±0.3 |
Difference of preferential interaction coefficients between associated and free proteins.
Regional preferential interaction coefficients at the protein-protein interface region inte(D) and the complementary protein surface region non-inte(D). The distance D is 7 Å for the D1.3-lysozyme complex and 9 Å for the D44.1-lysozyme complex.
Calculated from experimental KA-values at 6 molal glycerol (Figure 1).