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. 2013 May 16;9(5):e1003377. doi: 10.1371/journal.ppat.1003377

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© 2013 Jen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Colony immunoblotting of N. meningitidis C311#3 with anti-ChoP (TEPC-15) antibody revealed a colony with increased immunoreactivity and is indicated by the arrow. (B) Pilin was isolated from each of the mutants noted and examined under denaturing conditions by western blotting. No difference in TEPC-15 binding was observed between C311#3 WT, glycosylation mutants (C311#3pglE and C311#3pglA), or TEPC variants (C311#3TEPC+1 and +2). TEPC-15 did not bind to meningococci in which pili were not expressed (C311#3ΔpilE) or in which the ability of ChoP PTM was lost (C311#3pptA) (C). Colony immunoblotting of C311#3 glycosylation mutants shows that TEPC-15 reactivity increases as the pilin linked glycan decreases in size.