Figure 7. N. meningitidis pili-linked ChoP and glycan PTMs directly mediate an association with the PAFr.

Purified pili and recombinant human PAFr were used to examine a direct pili-PAFR interaction by a modified ELISA, as described within the text. Pili used were isolated from a panel of N. meningitidis WT and mutant strains, as noted, and as are described in Tbl S1. Recombinant PAFr was coupled to the G-proteins, G_iα₂β₁γ₂ (PAFr-G2) or G_iα₃β₁γ₂ (PAFr-G3), which served as stabilizing agents. Data shown were obtained simultaneously; however, for ease of discussion they are shown as separate panels. A) WT and the mutant derivatives of strains C311#3, MPJ11, MPJ24, MPJ26, and MPJ50 served as a source for isolated pili. B) WT and the mutant derivatives of strain 8013SB WT, served as a source for isolated pili.