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. 2013 May 16;9(5):e1003505. doi: 10.1371/journal.pgen.1003505

Figure 5. MiR-335 was constitutively overexpressed in RS7 and RS73 LCLs.

Figure 5

(A) RT-qPCR indicating >10-fold increases in miR-335 expression in RS7 and RS73 LCLs when compared to WT and A-T LCL controls. Three different WT LCLs were used in RT-qPCR experiments and were used to normalize the expression values for A-T, RS7, and RS73 LCLs. (B) Immunoblotting of cytoplasmic and nuclear lysates isolated from RS7 cells with or without 10 Gy IR indicated reductions in CtIP protein levels. Two bands were noted, representing modified and unmodified CtIP. (C) Treatment of RS7, RS73 and MCF7 cells (also miR-335 overexpressing) with AMO-miR-335 increased nuclear CtIP protein levels compared to WT and AT cells. The quantification of CtIP is shown below the blot after normalization to the loading control.