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. 2013 May 16;9(5):e1003505. doi: 10.1371/journal.pgen.1003505

Figure 6. MiR335-induced DDR defects were abrogated in RS7 and RS73 after AMO-miR-335 treatment.

Figure 6

(A) Partial restoration of BRCA1-IRIFs 8 hours post-12 Gy in constitutively miR-335 overexpressing RS7 and RS73 LCLs following AMO-miR-335 exposure (+). The (−) denotes similar treatment with AMO-CTL. RS67 is deficient for RNF168 and does not form stable BRCA1-IRIFs [27]; it was used as a negative control for BRCA1-IRIFs in these experiments. (B) Treatment with AMO-miR-335(+) partially corrected the reduced colony survival, a ‘radiosensitive’ cellular phenotype, after 1 Gy IR in RS7 and RS73. The RS cutoff is based on previous optimizations [23].