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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Yeast. 2013 Apr 12;30(5):191–200. doi: 10.1002/yea.2951

Figure 1.

Figure 1

A DMSO-based protocol produces highest transformation efficiencies and can be improved when cells are allowed to recover by incubation in broth. (A) Both one-step and DMSO-containing protocols generated higher transformation efficiencies than a simpler PEG/LiAc protocol. (B) Tris and EDTA are needed for maximum efficiency using the DMSO-based protocol. (C) Resuspension of cells after heat shock in YPDA broth, YP broth (lacking glucose and adenine), or phosphate-buffered saline (1×PBS) followed by immediate plating does not improve colony formation, but (D) incubation at 30 °C for 40 min in YPDA and YP media increased efficiency by 2-fold. Control, unmodified DMSO-based protocol. Error bars indicate standard deviations.