Table 2. Primers used and V. parahaemolyticus genomic regions investigated.
| ID | Chromose | Genome reference | Gene target | Primer Sequence (5′ - 3′) | Product size (bp) | Annealing T° (Extension time) | Associated genetic region |
| HGT1 | chr1 | RIMD 2210633 | VP0390 | TTTTCATCCATATCCATCGTCC | 579 | 62 (1 min) | (VPaI-1) |
| VP0392 | GCTTCCTCTTACCCTTTTCA | ||||||
| HGT1A | VP0389 | AAGCGGAGTCGTGTTTTTGTGT | 1723 | 64 (2 min) | |||
| VP0392 | TCCTGCCTTCGCGCTATCTTTG | ||||||
| HGT2 | chr1 | RIMD 2210633 | VP1072 | AAAGAGTTGACCGCTGTAAGG | 1677 | 62 (1.3 min) | (VPaI-3) |
| VP1073 | CGCTGTAACGTCATTGGTCTA | ||||||
| HGT3 | chr1 | RIMD 2210633 | VP1394 | ACGGCACCATCAGATTCATACCA | 1116 | 62 (1.3 min) | T6SS |
| VP1396 | ATAGCACCGCCCAATAGACCTGT | ||||||
| HGT3A | VP1408 | GAGATAACCGCACAACCAAG | 1065 | 62 (1.3 min) | |||
| VP1409 | CTGATCAAAAAACCGAGGCT | ||||||
| HGT4 | chr1 | RIMD 2210633 | VP1561 | GAGCGCTTCAAAGTGGGGATTAC | 1142 | 62 (1.3 min) | Phage f237 |
| VP1563 | CTGCCATTGGATTGATAGAAACC | ||||||
| HGT5 | chr1 | RIMD 2210633 | VP1796 | TGGGCTGAAAACAATAACCTCT | 1026 | 62 (1.3 min) | Super Integron |
| VP1798 | TGCTGCAGCCAAACTAATACC | ||||||
| HGT5A | VP1845 | TCGATCATAGGCCGCATAACAG | 1293 | 62 (1.3 min) | |||
| VP1848 | TTCGCATCCAACTTCATCAACC | ||||||
| HGT6 | chr1 | RIMD 2210633 | VP1886 | TGGGCTACGTCTTTGCTACTGA | 1577 | 62 (1 min) | cold-shock proteins, |
| VP1888 | ATACCATGATGCCAAAGAGACG | Ribonuclease R | |||||
| HGT6A | VP1889 | TGGTGGTGAAGACTTGTTTG | 594 | 62 (1 min) | |||
| VP1890 | TCTTTCTCTGGCTCGTCATT | ||||||
| HGT7 | chr1 | RIMD 2210633 | VP2139 | GAAGCGCGTTGGCAGGAATAC | 1066 | 64 (1.3 min) | |
| VP2142 | TGGAAGGGGGACGGATACGAT | (VPaI-4) | |||||
| HGT8 | chr1 | RIMD 2210633 | VP2903 | AAAGAAAGGTGGGAGGGTGAAG | 753 | 62 (1 min) | (VPaI-5) |
| VP2905 | TTTGACAAGGAGAACGGAGGTG | ||||||
| HGT9 | chr2 | RIMD 2210633 | VPA0434 | ATCTCAAACCCCCGCTCAAAAG | 770 | 62 (1 min) | putative site-specific |
| VPA0435 | TAAAAGACACCGCCTACGACTGC | recombinase | |||||
| HGT10 | chr2 | RIMD 2210633 | VPA1261 | GTTTTGCCACAGCCATTATTTC | 963 | 62 (1 min) | (VPaI-6) |
| VPA1262 | TTCCCTTTCGATCTCCAACACC | ||||||
| HGT12 | chr1 | RIMD 2210633 | VP0639 | GGGCATGTTAAAGCGTTAGT | 948 | 62 (1 min) | (VPaI-2) |
| VP0641 | TAGGTAAGTGTATCGAGCGT | ||||||
| HGT13 | chr1 | RIMD 2210633 | VP0657 | ACAGCGGCACAGAATATTAC | 792 | 62 (1 min) | type IV pilin |
| VP0658 | GTTGGCTCGTTTAATTTGGT | ||||||
| HGT14 | chr1 | RIMD 2210633 | VP1365 | AGGTTGTGAAAGGGGATTAG | 1010 | 62 (1.3 min) | hypothetical proteins |
| VP137 | GACAACAAACAACGAGACCA | ||||||
| HGT15 | chr1 | RIMD 2210633 | VP2693 | AACAATACGACGATCAGGAC | 856 | 62 (1 min) | MSHA pilin |
| VP2695 | TACACCACCGACAGGAATCA | ||||||
| HGT16 | chr2 | RIMD 2210633 | VPA0891 | AGCCAGAATACCCAAAGGGA | 1065 | 64 (1.3 min) | Phage related genes |
| VPA0893 | GCCACCAAACGAAAACACA | ||||||
| HGT16A | VPA0904 | GGAAACGGTGGCTTTTTAATG | 1051 | 62 (1.3 min) | |||
| VPA0908 | GAGCTTTGAGGGAGTGAAAT | ||||||
| HGT17 | chr2 | RIMD 2210633 | VPA0953 | CTCTACTAATTCAACGACCACT | 766 | 62 (1 min) | biofilm-associated surface |
| VPA0954 | CATATCTTTGCGGGTCATCT | proteins | |||||
| HGT18 | chr2 | RIMD 2210633 | VPA1564 | TAACCAAAAGAGCGCAAAAG | 859 | 62 (1.3 min) | hypothetical protein |
| VPA1565 | AACATCAAAACCAACACCAG | ||||||
| HGT19 | chr2 | RIMD 2210633 | VPA1702 | TCAAAGTGGTCGCAGCATAA | 1026 | 62 (1.3 min) | hypothetical protein |
| VPA1704 | AAGACCAATGGCGAAGAAA | ||||||
| HGT20 | chr1 | AQ3810 | A79_3286 | GGAAGCGAACAACATGAGAT | 922 | 62 (1.3 min) | Type I restriction system |
| A79_3288 | GAATGAAGAAATAGGTGAGGG | ||||||
| HGT20A | A79_3276 | CCACTTCTGCACATCTTCAA | 627 | 62 (1 min) | |||
| A79_3277 | GCATTTTTCTCTCGCCCATT | ||||||
| HGT21 | chr1 | AQ3810 | A79_2542 | CACCCTAAGCATATCAAACA | 701 | 62 (1 min) | Integron cassette |
| A79_2543 | GACAGCAGAAGAAATATCCA | ||||||
| HGT22 | chr1 | AQ3810 | A79_5185 | ATAGTCAGGTTTTCATCGGG | 941 | 62 (1 min) | hypothetical protein |
| A79_5186 | TTAAGGAAGAGGGGAAAGCA | ||||||
| T3SS2α | chr2 | RIMD 2210633 | VPA1338 | CGTCGTGGCGTGATCTCTACTT | 1083 | 64 (1 min) | T3SS2α (VPaI-7) |
| VPA1339 | ATTGCAGCAGCCATTACGAACA | ||||||
| VPA1355 | TTTAACCCCAACGATTCAGTGC | 709 | 62 (1 min) | ||||
| VPA1356 | CATGCGTAGTCAAGCCCGTTAT | ||||||
| T3SS2β | chr2 | AQ4037, | vscN | TTGGGGTGATTTCGACTTTT | 767 | 60 (1 min) | T3SS2β |
| TH3996 | vscC | GTGGCGTTTATGATCTTGTT | |||||
| vopD2 | GACGCCAATGAAATACCAAA | 1068 | 60 (1 min) | ||||
| vopB | CAGAAGAAGGCAGAACAAAA |