Figure 3. The temperature-sensitive period for pmr-1(ru5) embryos is during anterior, C-lineage, and ventral cell migrations.

In the upshift experiments, embryos, extracted from gravid adults grown at the permissive temperature (15°C) from the mid-L4 stage, were switched to the restrictive temperature (25°C) at the indicated time. Embryos were then maintained at the restrictive temperature for the duration of embryogenesis and scored “viable” if they thrived past the L1 stage. In the downshift experiments, the same protocol was followed except that the embryos were switched from restrictive temperatures (25°C) to permissive temperatures (15°C) at the indicated times. The lines below the graph correspond to the times when anterior, C-derived, and ventral lineage cell migrations were assayed. All times were normalized to correspond to development at 25°C; n = 5 to 36 embryos at each time point.