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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Calcif Tissue Int. 2013 Mar 17;92(6):576–585. doi: 10.1007/s00223-013-9720-z

Fig 3.

Fig 3

Isolation of osteocyte dominant RNA. A, Representative photomicrographs of H&E-stained sections of the tibiae where a bone flush (top) and filing technique (bottom) were used. Remnants of the BM was obvious in the flushed tibia but not in the filed tibia. B, Total RNA was isolated from the filed cortical bone and qPCR performed to verify osteocyte dominant RNA by evaluating SOST and BM dominant RNA by assessing cxcl12 expression. C, Serum calcium levels at 12 weeks were plotted. 3-month ZA-treatment significantly decreased the serum calcium levels. A single injection of PTH increased the serum calcium levels significantly in the VC-treated mice but not in the ZA-treated mice. n>5/group, *P < 0.05, **P < 0.01, ***P < 0.001.