Fig 5.
ZA treatment influenced the gene expression of BM cells in response to acute PTH. Total RNA was isolated and qPCR was performed. Results normalized to GAPDH are presented. Apoptosis-related genes (Bim, BAD, BAK, and Bcl2), MMP-13, and VEGFA were analyzed. A, The effect of 3-months ZA and VC treatment on the gene expression of the BM were assessed. B, The responses of the BM to acute PTH over saline were plotted for mice on 3-month VC and ZA treatment. The value in the plot is greater than one when acute PTH upregulated the gene expression of the BM. n=5/group, *P < 0.05, **P < 0.01, ***P < 0.001