Table 2.

The 20 ISSR primers used for genetic diversity analysis of safflower accessions. Observed number of alleles (Na), Effective number of alleles (Ne), Nei’s (1973) gene diversity (h), Shannon’s Information index (I). P, number of polymorphic loci

Primer No.	Primer sequence	Na	Ne	H	I	P	% P
1	5′-BDB(TCC)5-3′	1.7500	1.3791	0.2402	0.3715	12	75.00
2	5′-HVH(TCC) 5-3′	1.7500	1.5280	0.3012	0.4404	12	75.00
3	5′-DBD(AC) 7-3′	1.8750	1.5057	0.3065	0.4628	14	87.50
4	5′- (TCC) 5RY-3′	1.9333	1.4527	0.2699	0.4143	14	93.33
5	5′-VHVG(TG) 7-3′	1.6667	1.2948	0.1908	0.3013	8	66.67
6	5′- (AG) 8YC-3′	2.0000	1.7226	0.4015	0.5842	18	100.00
7	5′- (AC) 8YG-3′	2.0000	1.7226	0.4015	0.5842	18	100.00
8	5′- (AG) 8T-3′	1.8571	1.5055	0.2993	0.4505	12	85.71
9	5′- (AG) 8C-3′	1.7647	1.3367	0.2119	0.3325	13	76.47
10	5′- (GA) 8T-3′	1.7059	1.4457	0.2590	0.3857	12	70.59
11	5′- (GA) 8C-3′	1.8095	1.4586	0.2793	0.4226	17	80.95
12	5′- (CT) 8G-3′	1.8667	1.5280	0.3039	0.4540	13	86.67
13	5′- (CA) 8G-3′	1.7647	1.5749	0.3183	0.4597	13	76.47
14	5′- (TC) 8C-3′	2.0000	1.5520	0.3291	0.5000	18	100.00
15	5′- (TC) 8G-3′	1.7143	1.3294	0.2088	0.3252	10	71.43
16	5′- (AC) 8C-3′	1.9048	1.6003	0.3396	0.4997	19	90.48
17	5′- (TG) 8G-3′	1.9375	1.6153	0.3554	0.5251	15	93.75
18	5′- (AG) 8YT-3′	1.8889	1.4057	0.2523	0.3941	16	88.89
19	5′- (GA) 8YC-3′	1.7500	1.4760	0.2793	0.4158	15	75.00
20	5′- (CT) 8RG -3′	1.8333	1.3338	0.2136	0.3390	15	83.33

R=A/T, Y=G/C, B=T/G/C, D=A/T/G, H=A/T/C, V=3A/G/C