Table 1.

Effect of concentrations of auxins (2,4-D and NAA) on callus induction in Sarcostemma acidum on MS medium + additives

Name of PGR	Concentration of PGR (mg L−1)	Callus intensity	Remarks
Control	0.0	−	No response
2,4-D
	0.5	−	No response
	1.0	+	Slow growing callus
	1.5	+	Slow growing, watery callus
	2.0	++	Slow growing and fragile callus
	3.0	++	Brown and fragile callus
	4.0	++	Limited growth, brown, fragile callus
	5.0	+	Explant become brown, Poor growth
NAA
	0.5	+	Slow response, non-regenerative callus
	1.0	+	Non-regenerative callus
	1.5	+	Non-regenerative callus
	2.0	+++	White, granular and fast growing callus
	3.0	+++	Fast-growing, fragile callus
	4.0	++	Slow growing and fragile callus
	5.0	++	Browning of callus

+ = Slow; ++ = Moderate; +++ = High