Table 3.

Effect of infection by Metarhizium anisopliae on reproduction potential of Glossina morsitans morsitans females

Treatment	1st line females	2nd line females	3rd line females
No. fertile females (X ± SE)
Fungus-free (control)	7.5 ± 0.6a	7.5 ± 0.6a	6.0 ± 0.9a
M. anisopliae	3.4 ± 0.5b	3.5 ± 0.9b	6.0 ± 1.1a
t	6.92	5.90	N/A
P	0.001	0.005	N/A
No. pupae/female (X ± SE) per female produced between 1 and 35 days after infection 1
Fungus-free (control)	2.3 ± 0.2a	2.5 ± 0.2a	2.0 ± 0.1a
M. anisopliae	1.2 ± 0.1b	1.4 ± 0.2b	1.4 ± 0.3a
t	2.82	3.0	1.32
P	0.030	0.025	0.245
No. pupae (X ± SE) 2
Fungus-free (control)	4.0 ± 0.5a	3.5 ± 0.5a	3.5 ± 0.6a
M. anisopliae	1.4 ± 0.3b	2.5 ± 0.9a	2.1 ± 0.4b
t	4.2	1.41	4.37
P	0.013	0.227	0.011

Four replicates of 10 flies each. Female flies mated with Metarhizium anisopliae-treated and fungus-free male ‘donor’

¹Number of pupae/fly produced by fungus-treated flies that survived up to 35 days post-infection, and free-fungus flies for the same period

²Number of pupae produced per female over a period of 60 days post-infection. Means within-column followed by the same letter are not significantly different by t test (P > 0.05)