Fig. 2.

Evaluation of the resolution properties of the MonoS 5/50 GL column used to separate bovine lactoferrin in a two-step purification. a MonoS chromatography of DMV International lactoferrin (the collected fraction highlighted in gray bar). b Fraction obtained from the first ion exchange separation was modified using the methods described in experimental section to get iron-saturated and iron-depleted lactoferrin forms. c Elution profiles of apo- and hololactoferrins from the MonoS column from the second separation: apo-Lf (black), holo-Lf (red)