Fig. 4.

Tubulin and actin identify cilia and microvilli, respectively. a Antibodies against tubulin and a marker of f-actin (phalloidin) both labeled structures in the whole MOE and in the adjacent non-sensory epithelium (NSE; tubulin, left-hand panel; f-actin, right-hand panel). b Higher magnifications of the apical MOE show cilia labeled with antibodies against tubulin (left-hand panel), microvilli labeled with phalloidin (middle panel), and a double-labeled MOE (right-hand panel). c ORNs and their processes were visualized by nerve backfills with biocytin and double labeled with antibodies against tubulin (left panel, ciliated neuron, the arrow points to cilia), and phalloidin (middle and right panels, microvillous neurons, arrows point to olfactory knobs). Another backfilled neuron with somewhat longer processes (asterisk) was not labeled by phalloidin, i.e., it is a ciliated neuron