Table 1.
Colony growth on agar medium resulting from bacterial and fungal cells recovered from interaction samples
Fungal treatmenta | Interaction sampling timeb | |||||
---|---|---|---|---|---|---|
24 h | 48 h | 72 h | ||||
B | F | B | F | B | F | |
No bacteria w/antibiotic | − | + | − | + | − | + |
No bacteria w/o antibiotic | − | + | − | + | − | + |
C3 w/antibiotic | − | + | − | ± | − | − |
C3 w/o antibiotic | + | − | + | − | + | − |
DCA w/antibiotic | − | + | − | + | − | + |
DCA w/o antibioticc | + | + | + | + | + | + |
No bacteria untreated control, C3 L. enzymogenes wt strain C3, DCA L. enzymogenes mutant strain DCA
aTreatments supplemented with (w/) or without (w/o) the antibiotics gentamycin (3.6 mg/ml) and chloramphenicol (3.6 mg/ml)
bObserved growth (+) or no growth (−) of Lysobacter enzymogenes (B) and Cryphonectria parasitica (F) after plating mycelial fragments recovered from interaction samples on 10 % TSA. ± represents growth in some but not all treatment replicates
cFor all samples, mixed culture growth of both fungal and bacterial colonies were observed