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Tricine SDS-PAGE supplemented with glycerol for purified antimicrobial peptide AN5-1 produced by Paenibacillus alvei AN5. Lane 1 color marker ultra-low range (M.W. 1.06–26.6) KDa (Sigma, USA), lane 2 purified AN5-1 stained with Coomassie blue, lane 3 bacteriocin assay overlying on soft agar shows inhibitory activity of the active peptide against E. coli ATCC 29522
