Table 4.

Stability of purified antimicrobial peptide AN5-1 (80 AU ml⁻¹) against temperature, pH and chemicals using E. coli ATCC 13076 as indicator strain

Stability	Inhibition zones diameter
Temperature (°C)
40	+++
50	+++
60	+++
70	+++
80	+++
90	+++
100	++
110	–
pH values
1	–
2	+
3	+++
4	+++
5	+++
6	+++
7	+++
8	+++
9	+++
10	+++
11	+++
12	++
13	–
Chemicals
SDS	+++
EDTA	+++
Urea	+++

–, no inhibition zones; +, zone diameter 1–11 mm; ++, zone diameter 12–16 mm; +++, zone diameter 16–21 mm; ++++, zone diameter ≥22 mm