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Reconstitution of the Fe2III-Y• cluster and RNR activity in permeabilized Δrnr4 cells by the addition of recombinant β′ and β′Δ8aa. A, EPR analyses of permeabilized Δrnr4 cells in the presence of exogenous His6-β′ (green), His6-β′Δ8aa (red), or no protein addition (blue). B, specific activities of ββ′ were assayed using permeabilized Δrnr4 cells in the presence of exogenous His6-β′ (filled squares) and α, His6-β′Δ8aa (open squares) and α, or without any added protein (open circles).
