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DomPred predicts a domain boundary of LaeA near amino acid 100. A, full-length LaeA amino acid sequence was inputted to the DomPred domain prediction server. The output “aligned termini profile” predicts that LaeA has two domains split near amino acid residue 100. B, because the first motif of the AdoMet-binding site starts at amino acid 141, we chose to make N-terminal truncation mutants by removing 30, 42, or 86 residues.
