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. 2013 Apr 4;288(20):14135–14146. doi: 10.1074/jbc.M112.445643

FIGURE 1.

FIGURE 1.

Knockdown of Abi1 in X. laevis affects eye development. A, knockdown of Abi1 by injection of Abi1 MO (37.5 ng) into both blastomeres of two-cell stage embryos results in defects in eye development; injecting STD MO (37.5 ng) does not. Co-injection of abi1Δ5′UTR RNA (150 pg) largely restores normal eye development. B, Western blot to assess Abi1 MO efficacy and specificity. Xenopus oocytes were injected with abi1-HA RNA (10 ng) or abi1Δ5′UTR RNA (10 ng), alone or with Abi1 MO (10 ng). Wild-type abi1 RNA is susceptible to the MO, whereas abi1Δ5′UTR is resistant. C, whole mount in situ hybridization of stage 34 embryos using an abi1 antisense probe reveals expression in eye (lateral view and sagittal section). D, injection of Abi1 MO (10 ng) into the D1.1.1 blastomere of 32-cell stage embryos results in defects in eye development; injecting STD MO (10 ng) does not. Co-injection of abi1Δ5′UTR RNA (150 pg) largely restores eye development. E, summary of effects of 32-cell stage injections in D on eye development. Error bars represent mean ± S.E. F, sagittal sections of stage 37 embryos injected with Abi1 MO or STD MO along with Alexa 488-dextran (3.75 ng) into D1.1.1 blastomere. Note that the fluorescent cells containing Abi1 MO are found at the midline but not in the presumptive eye area.