Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 1;288(20):14238–14246. doi: 10.1074/jbc.M113.465484

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Tests for purity and activity of the FixK₂(C183S)-His₆ protein. a, Coomassie Blue-stained gel after SDS-PAGE of purified N-terminally and C-terminally His₆-tagged FixK₂(C183S) proteins. The N-terminally tagged construct copurified with a truncated version (His₆-FixK₂(C183S)-220) that lacks the 12 C-terminal amino acids. b, IVT activation assay with 0.1–1 μm FixK₂(C183S)-His₆ added to the test mixture. A plasmid template (pRJ8816) containing the FixK₂-dependent fixN promoter cloned upstream of a strong transcription terminator was used for multiple-round IVT with RNA polymerase holoenzyme from B. japonicum. A FixK₂-independent transcript encoded by the plasmid served as a useful internal control for the RNA polymerase activity. The two left lanes contain RNA size markers with lengths of 180 (M) and 286 (M′) nucleotides.