FIGURE 1.

IleRS is essential for T. brucei growth and infection. a, cumulative growth curve of T. brucei bloodstream forms with tetracycline-inducible RNAi against IleRS. Tet (+), RNAi induced with 1 μg/ml tetracycline; Tet (−), non-induced. b, parasitemia of mice infected with the T. brucei bloodstream form IleRS RNAi line. Mice were infected with 1.0 × 10⁴ parasites. Mice received doxycycline (Dox; 200 μg/ml) added to the drinking water for RNAi induction 18 h before infection (b.i.) or 24 or 48 h post-infection (p.i.) and replaced daily. Non-induced mice received water without doxycycline. Data in a are the mean ± S.D. of triplicate measures, and those in b are the mean ± S.D. of two experiments performed in triplicate.