FIGURE 7.

In vitro flavinylation of apoNqrC′. The apoNqrC′ protein (4 mg/ml) was incubated in medium containing ApbE′ (0.1 mg/ml), MgSO₄ (5 mm), and flavin (1 mm), as indicated under the gel. The reaction products (5 μl) were separated by SDS-PAGE. A, Coomassie-stained gel. B, unstained gel under UV illumination. The bars with numbers on the left side refer to the positions and molecular masses of marker proteins.