Skip to main content
. 2013 Mar 28;288(20):14400–14407. doi: 10.1074/jbc.M113.464834

FIGURE 4.

FIGURE 4.

The release of free P-TEFb from the 7SK snRNP is also observed with HDACis that reactivate HIV in HeLa cells. A, HDACis that reactivate HIV also disrupt the 7SK snRNP as determined by RNA immunoprecipitation in HeLa cells. HeLa cells were treated with HDACis (SAHA, 5 μm; MS-275, 25 μm; ST-80, 30 μm; TBSA, 20 μm) for 4 h, and CDK9 was immunoprecipitated from cell lysates. CDK9-associated 7SK snRNA in immunoprecipitations was determined by RT-qPCR. Values are presented as relative enrichment with the DMSO control set to 1. Error bars represent S.E. of mean of experiments performed in triplicate. B, these HDACis also activate a Hex.Luc plasmid target in HeLa cells. Hex.Luc was transfected into HeLa cells. After 24 h, cells were treated with HDACis (SAHA, 5 μm; MS-275, 5 μm; ST-80, 30 μm; TBSA, 5 μm) for 24 h, and luciferase activity was measures in cell lysates. Values are presented as -fold activation over the DMSO control set to 1 (bar 1). Error bars represent S.E. of mean of experiments performed in triplicate.