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Genetic complementation of pyruvate auxotrophic yeast by T. brucei cDNA clones. A, clones A, B, C, and JMY75 pyruvate auxotrophic yeast (top quadrant of plate) were grown on solid YNB medium supplemented with Trp, His, 2% ethanol, and 10 mm pyruvate and incubated at 30 °C for 4 days. B, TbPT0 cDNA and predicted amino acid sequence. The cDNA sequence, including the splice leader sequence (SLS), 5′-UTR, ORF, 3′-UTR, poly(A+) tail, and predicted amino acid sequence, are depicted.
