FIGURE 6.

Inhibition of the TbPT gene family expression by RNAi. T. brucei BF/1313 clone transfected with the hairpin loop TbPT-RNAi construct was incubated in culture medium containing 1 μg ml⁻¹ doxycycline (+dox; closed circles) or without doxycycline (−dox; open circles). A, growth curve of BF/TbPT-RNAi clone starting with 10⁵ parasites ml⁻¹. Y axis represents the log₁₀ of cumulative cell ml⁻¹, and the x axis represents time (h). B, Northern blot of 5 μg of total RNA isolated from BF/TbPT-RNAi or BF/1313 grown for 48 h in the presence (+) or absence (−) of 1 μg ml⁻¹ doxycycline, hybridized with TbPT0 (upper panels) and α-tubulin (lower panels).