FIGURE 7.

His₆-RbpA binds and forms a tight complex with His₆-σ^B_1–228. A, elution profiles from a size exclusion column (Superdex 75 10/300) of His₆-RbpA or His₆-σ^B_1–228 individually and the complex formed between them (1:1 molar ratio). The elution volumes of the protein molecular mass standards are indicated by dashed lines. B, samples from the fractions collected during the different size exclusion chromatography runs were analyzed by SDS-PAGE. Lane MW shows molecular mass markers, lane L shows the column load, and lanes 8–12 are labeled according to the elution volume (ml).