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. 2013 Apr 5;288(20):14451–14462. doi: 10.1074/jbc.M113.460840

FIGURE 3.

FIGURE 3.

The p53-p21 and p57-E2F7 pathways are reduced in DEN-treated mice. A, protein and RNA levels of p53 are reduced in DEN-treated mice. Western blotting was performed with nuclear extracts from livers at different time points after DEN injections. The filter was reprobed with Abs to β-actin, and the levels of p53 were calculated as ratios to β-actin and then as percentages of the 0 time point (center panel). The bottom panel shows quantitative RT-PCR with mRNA isolated from livers at different time points after DEN treatments. Bar graphs show the results of analyses of three animals of each group. *, p < 0.05; **, p < 0.01; DEN treatment group versus control group. B, protein and RNA levels of p21 and E2F7 are reduced in DEN-treated mice. The experiment was performed as described above for p53. Bar graphs show the results of analyses of three animals of each group. *, p < 0.05, DEN treatment group versus control group; #, p < 0.05, DEN treatment group versus control group. C, liver proliferation is increased in DEN-treated mice. BrdU uptake was performed with livers isolated from mice at 20, 25, 30, and 35 weeks after DEN injections. The top panels show typical pictures of BrdU staining. The bottom panel shows percentages of BrdU-positive hepatocytes as an average of data with five animals for each time point.