FIGURE 2.
Inhibition of amino acid transamination by AOA blocks caspase activation. A, egg extracts supplemented with AOA (10 mm) or buffer were analyzed for caspase-2 activity at the indicated time points using the BioVision caspase-2 colorimetric assay kit and measuring cleavage of the caspase-2 model substrate, VDVAD-pNA, spectrophotometrically. B, 35S-labeled caspase-2 was incubated in AOA- or mock-treated extracts, and samples were resolved by SDS-PAGE/PhosphorImager. C, egg extracts treated as in A were analyzed for caspase-3 activity at the indicated time points using the caspase substrate Ac-DEVD-pNA. RT, room temperature.