FIGURE 2.

JIP3 enhances axon elongation in primary hippocampal neurons. A, PC12 cells were transiently transfected with scramble, JIP3-EGFP, siJIP3, or siJIP3 + rJIP3-EGFP construct. At 72 h after transfection, cells were lysed and immunoblotted with anti-JIP3 and anti-α-tubulin antibodies. B, percentages of electroporated hippocampal neurons in stage 1, stage 2, and stage 3 when cultured for 24 or 36 h (n = 3). C, hippocampal neurons (DIV5) transfected with the indicated construct were immunostained with anti-GFP (green) and anti-α-tubulin (red) antibodies. Arrows point to the GFP-positive neurons. Scale bar, 40 μm. D and E, quantitation of the length of axons (D) or dendrites (E) shown in C; >100 cells were examined in each experiment (n = 3, *, p < 0.05, versus the scramble group; Student's t test).