FIGURE 2.

VitD enhances DEX induction of MKP-1 in CD14⁺ cells and U937 cells monocytic cell line only in the presence of CD14⁻ cells. A, purified CD14⁺ cells (n = 12); B, purified CD14⁻ cells (n = 12); C, CD14⁺ cells with CD14⁻ cells in tissue culture inserts (n = 4); D, CD14⁻ cells in tissue culture inserts with CD14⁺ cells on the bottom of the cell culture wells (n = 4); E, U937 cells with CD14⁻ cells in tissue culture inserts (n = 3) were preincubated with VitD for 21 h, followed by DEX (10 nm) treatment for 3 h. MKP-1 mRNA levels were detected by real-time PCR and were normalized to β-actin mRNA. Values represent mean ± S.E. (error bars).