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Nonconjugatable SUMO does not co-localize with GFP-Nrf2 or with endogenous PML protein. HepG2 cells grown as in Fig. 3 were co-transfected with 2 μg of pEGFP-Nrf2 with 0.5 μg of expression plasmid for RFP-SUMO-1ΔGG (A and C) or RFP-SUMO-2ΔGG (B and D); the cells in C and D were immunostained for endogenous PML protein, as described under “Experimental Procedures.” Scale bars, 10 μm.
