FIGURE 5.

Overexpression of CRT increases TGF-β stimulation of ECM. Parental and CRT overexpressing L fibroblasts were grown overnight in medium with 10% FBS, starved for 2 h in medium with low (0.5%) serum, and treated with 100 pm TGF-β for 24 h for fibronectin determinations (A) or 48 h for collagen I determinations (B). Cells were harvested with Laemmli buffer, separated by SDS-PAGE, and immunoblotted for fibronectin, collagen I, and β-tubulin. C, bands were analyzed by densitometry (n = 3 separate experiments) and normalized to β-tubulin ±S.D. Untreated cells were set to 1.0. *, p < 0.05 versus parental cells. CRT overs, CRT overexpressors.