TABLE 1.
Parameters of SRM assays of NIK, TRAF1, and p52/p100
m/z values listed are for the natural forms of the peptides.
| Gene name | UniProt accession no. | Peptide sequence | Peptide molecular mass | Precursor ion (m/z) | Product ion (m/z) | Ion type | Collision energy |
|---|---|---|---|---|---|---|---|
| Da | V | ||||||
| TRAF1 | Q13077 | VVELQQTLAQK | 1255.721 | 628.864 | 688.398 | y6 | 25 |
| 628.864 | 816.457 | y7 | 25 | ||||
| 628.864 | 929.541 | y8 | 25 | ||||
| 628.864 | 1058.583 | y9 | 25 | ||||
| NIK | Q99558 | IASEPPPVR | 964.541 | 483.274 | 468.292 | y4 | 20 |
| 483.274 | 565.345 | y5 | 20 | ||||
| 483.274 | 694.388 | y6 | 20 | ||||
| 483.274 | 781.420 | y7 | 20 | ||||
| p52/p100 | Q00653 | DSGEEAAEPSAPSR | 415–428 | 701.808 | 359.203 | y3 | 27 |
| 701.808 | 614.325 | y6 | 27 | ||||
| 701.808 | 743.368 | y7 | 27 | ||||
| 701.808 | 814.405 | y8 | 27 | ||||
| 701.808 | 885.442 | y9 | 27 | ||||
| 701.808 | 1014.484 | y10 | 27 |