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. 2013 Mar 29;288(20):14612–14623. doi: 10.1074/jbc.M113.464081

TABLE 1.

Parameters of SRM assays of NIK, TRAF1, and p52/p100

m/z values listed are for the natural forms of the peptides.

Gene name UniProt accession no. Peptide sequence Peptide molecular mass Precursor ion (m/z) Product ion (m/z) Ion type Collision energy
Da V
TRAF1 Q13077 VVELQQTLAQK 1255.721 628.864 688.398 y6 25
628.864 816.457 y7 25
628.864 929.541 y8 25
628.864 1058.583 y9 25
NIK Q99558 IASEPPPVR 964.541 483.274 468.292 y4 20
483.274 565.345 y5 20
483.274 694.388 y6 20
483.274 781.420 y7 20
p52/p100 Q00653 DSGEEAAEPSAPSR 415–428 701.808 359.203 y3 27
701.808 614.325 y6 27
701.808 743.368 y7 27
701.808 814.405 y8 27
701.808 885.442 y9 27
701.808 1014.484 y10 27