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Mutagenesis primers used in the study
Modified codons are underlined.
| Primer | Nucleotide sequence 5′ to 3′ |
|---|---|
| E177Aforward | GGGAACTTGCCAACGCGCCCAGGTGCAAGGG |
| E177AReverse | CCCTTGCACCTGGGCGCGTTGGCAAGTTCCC |
| E283AForward | CCGTGTTTGTTGGAGGCGTATGGGTATGAGAGTGATAGG |
| E283AReverse | CCTATCACTCTCATACCCATACGCCTCCAACAAACACGG |
| E320AForward | GGAGACCTCTTCACGCCGCGGAGGGTGGTTGG |
| E320AReverse | CCAACCACCCTCCGCGGCGTGAAGAGGTCTCC |
| E410AForward | GATGATTGCTGCTGCAGCCGTTGGCGCTGCC |
| E410AReverse | GGCAGCGCCAACGGCTGCAGCAGCAATCATC |
