Figure 4.

p53 family members contribute to regulation of the autophagy program. (A) Table showing which of the p53-bound autophagy genes are bound by p63, based on a ChIP-seq study in primary human keratinocytes (Kouwenhoven et al. 2010), and by p73, based on a ChIP–Chip study in human rhabdomyosarcoma cells (Rosenbluth et al. 2011). (B) qRT–PCR analysis of autophagy gene expression in untreated (ut) or doxorubicin-treated (dox) wild-type and p53^−/− MEFs transfected with either control (ctr) or p63 and p73 siRNAs. Expression levels represent the average ± SD of technical triplicates after normalization to expression in untreated cells and to β-actin.