Skip to main content
. 2013 May 1;27(9):991–996. doi: 10.1101/gad.211243.112

Figure 2.

Figure 2.

The pre-miR-134 terminal loop is necessary and sufficient for dendritic targeting. (A) Sequences of in vitro transcribed pre-miR-134 (blue) and pre-miR-150 (gray). The five central nucleotides of the terminal loop are shown in bold. Sequence changes required for efficient in vitro transcription (G at position +1) and for correct 3′ termini (2-nt overhang) are indicated in black. (B) Representative images of hippocampal neurons transfected with indicated Cy3-pre-miRNAs. (Left) Cy3-pre-miRNA (red) and Hoechst (blue). (Middle) MAP2 (green). (Right) Merge. Arrows and arrowheads indicate pre-miRNA puncta localized in dendrites or the cell body, respectively. Bar, 10 μm. (C–E) Percentage of dendritic puncta for the indicated pre-miRNAs or mutants (mean ± SD, n = 4, 64 neurons per condition). (L) Loop; (C) central loop.