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. 2013 May 1;27(9):997–1002. doi: 10.1101/gad.215152.113

Figure 4.

Figure 4.

Smad2-dependent Eomes and Lhx1 expression in the EmVE. Localization of Eomes, Lhx1, and Foxa2 in E5.5 embryos of the indicated genotypes. (A–A3) In wild type (WT), Eomes is present in the ExE and EmVE. Lhx1 colocalized with Eomes in EmVE only. (B–B3) In Smad2−/− mutants, EmVE expression of Eomes and Lhx1 is lost, while Eomes expression in ExE is maintained. (C–C3) In the absence of Lhx1, EmVE Eomes is present. (D–D3) Foxa2 is not required for the expression of Eomes and Lhx1. (E–E3) In wild type, Foxa2 is expressed throughout the VE. Foxa2 expression is unchanged in Smad2−/− (F–F3) and Lhx1−/− (G–G3) mutants. (H–H3) Lack of Foxa2 does not alter expression of Eomes in EmVE. (I–I3,J–J3) Absence of Lhx1 in EomesΔVE/− mutant.